Data to support Fig 3 of the manuscript "Wide dynamic range of cellular flow through early αβ T-cell selection in the thymus"

2018-09-14T03:12:17Z (GMT) by Stephen Daley Rushika C. Wirasinha
<p>The file contains mouse T cell receptor (TCR) sequences obtained by multiplex PCR amplification of cDNA molecules followed by Illumina sequencing. Sequences were aligned to the mouse genome using MIGEC software (see doi: 10.1038/nmeth.2960 for details). Sequences with a read count of <3 were excluded. Except for the header row, each row contains information about a unique TCR nucleotide sequence. Columns 1-11 contain output from MIGEC software. Columns 12-15 contain information about sample origin, detailed as follows: column 12 "sample_id" is an identifier for the sample of origin; column 13 "tcr_b" specifies the TCR beta chain status of donor mouse ("poly" = C57BL/6 inbred mouse strain); column 14 “mouse” is an identifier for the individual mouse of origin and column 15 "subset" specifies the T cell subset of origin ("t_1" = Type A IELp thymocytes; "t_4" = CD4+ naive thymocytes; "t_8" = CD8+ naive thymocytes). For materials and methods refer to Wirasinha et al Immunol Cell Biol. 2018 Jul;96(6):553-561. doi: 10.1111/imcb.12047. PMID: 29726044.</p>