DNA binding and cytotoxicity studies of magnetic nanofluid containing antiviral drug oseltamivir

<p>In this work, the possibility of preparing a nanoparticle with improved treatment properties was investigated. In this regard, synthesis, characterization, in vitro cytotoxicity and DNA binding of Fe<sub>3</sub>O<sub>4</sub>@oleate/oseltamivir magnetic nanoparticles (MNPs) were investigated. Fe<sub>3</sub>O<sub>4</sub> nanoparticles were synthesized via chemical co-precipitation and coated by oleate bilayers. Then, Fe<sub>3</sub>O<sub>4</sub>@OA MNPs were functionalized with an antiviral drug (oseltamivir), for better biological applications. The MNPs were subsequently characterized by zeta sizer and Zeta potential measurements, Fourier transform infrared (FT-IR) spectroscopy, vibrating sample magnetometer (VSM) and transmission electron microscopy (TEM) analyses. The TEM image demonstrated that average sizes of Fe<sub>3</sub>O<sub>4</sub>@OA/oseltamivir MNPs were about 8 nm. The in vitro cytotoxicity of Fe<sub>3</sub>O<sub>4</sub>@OA/oseltamivir MNPs was studied against cancer cell lines (MCF-7 and MDA-MB-231) and compared with oseltamivir drug. The results illustrated that Fe<sub>3</sub>O<sub>4</sub>@OA/oseltamivir magnetic nanoparticles have better antiproliferative effects on the mentioned cell lines as compared with oseltamivir. Also, in vitro DNA binding studies were done by UV–Vis, circular dichroism, and Fluorescence spectroscopy. The results indicated that Fe<sub>3</sub>O<sub>4</sub>@OA/oseltamivir MNPs bound to DNA via groove binding. Moreover, this magnetic nanofluid has potential for magnetic hyperthermia therapy due to magnetic core of its nanoparticles.</p> <p>Communicated by Ramaswamy H. Sarma</p>