Fig 7.TIF (1.26 MB)
DENV NS1 induces VE-cadherin disarray, which is rescued by inhibiting MIF or autophagy.
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posted on 2016-07-13, 16:10 authored by Hong-Ru Chen, Yung-Chun Chuang, Yee-Shin Lin, Hsiao-Sheng Liu, Ching-Chuan Liu, Guey-Chuen Perng, Trai-Ming Yeh(A) HMEC-1 cells were treated with PBS, 293T-NS1, 293T-NS1 mixed with ISO-1, 293T-NS1 mixed with 3-MA, ISO-1 only or 3-MA only. After 6 h, the cells were fixed, and IFA was applied by anti-VE-cadherin antibodies. The fluorescence images were acquired by a FV1000 confocal microscopy. The fluorescent intensity was quantified by Image J software and cytosolic/perinuclear versus barrier/marginal VE-cadherin ratio is shown in (B) by measuring 50 cells for each condition. *P<0.05, **P<0.01, ***P<0.001.
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DENV NS 1-induced LC 3 punctaLC 3-II conversionMIF secretionanti-NS 1 antibodiesDENV nonstructural protein 1DENV NS 1-inducedleakageDENV NS 1cell line HMECp 62 degradationDengue Virus Nonstructural Protein 1 Induces Vascular LeakageDSSMIF inhibitorsdengue hemorrhagic feverAtg 5 knockdown clonedengue shock syndromecytokine macrophage migrationAutophagy Dengue virusDENV NS 1-induced autophagyDHFMacrophage Migration Inhibitory Factor
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