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DBLβ12 domain of PFD0020c is involved in the interaction with gC1qR.

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posted on 2016-11-11, 04:30 authored by Ariel Magallón-Tejada, Sónia Machevo, Pau Cisteró, Thomas Lavstsen, Pedro Aide, Mercedes Rubio, Alfons Jiménez, Louise Turner, Aida Valmaseda, Himanshu Gupta, Briegel De Las Salas, Inacio Mandomando, Christian W. Wang, Jens E. V. Petersen, Jose Muñoz, Joaquim Gascón, Eusebio Macete, Pedro L. Alonso, Chetan E. Chitnis, Quique Bassat, Alfredo Mayor

A) Reactivity of PFD0020c domains against gC1qR and EPCR by ELISA-based binding assays. Antibody-mediated inhibition of B) binding to gC1qR or CD36 of P. falciparum Pf3D7gC1qR, Pf3D7CD36; C) binding to human brain endothelial cells (HBMEC) of Pf3D7gC1qR and D) binding to gC1qR of four P. falciparum Mozambican isolates. The gC1qR binding levels in absence of antibodies were 299 IEs/mm2 (SD 53) for Pf3D7gC1qR; 202 IEs/mm2 (SD 11) for Pfmoz1; 92 IEs/mm2 (SD 17) for Pfmoz2; 74 IEs/mm2 (SD 8) for Pfmoz3; and 81 IEs/mm2 (SD 7) for Pfmoz4. The CD36 binding level in absence of antibodies was 615 IEs/mm2 (SD 27) for Pf3D7CD36. Binding is expressed as the percentage of mean binding in absence of antibodies. Bars represent the mean and standard deviation. * indicates P<0.05 and **P<0.001.

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