Fig 4.tif (672.71 kB)
Cyclic stretch induced increase in cytosolic and nuclear levels of IL-33.
figure
posted on 2017-09-12, 17:41 authored by Jing Chang, Yuefeng Xia, Karla Wasserloos, Meihong Deng, Kory J. Blose, David A. Vorp, Heth R. Turnquist, Timothy R. Billiar, Bruce A. Pitt, Ma-Zhong Zhang, Li-Ming ZhangNuclear and cytoplasm protein fractions of MLE-12 cells with (cyclic S) or without (con) 6h stretch were isolated, and mIL-33 in nucleus and cytoplasm were measured by Western blot (A) or ELISA (B). Lamin B and β-actin (A) served as a loading control for nucleus and cytoplasmic protein, respectively. **P<0.01, ***P<0.001 compared with control (con). Each stretching group collected from three wells for a single experiment, the bar graphs illustrate data representative of three independent experiments.
