Cultivation in shake flasks with methanol feeding without (A, B) or with (C, D) manual pH adjustment

Cultivations were performed in two phases: initial batch phase in BMG-medium and fed-batch in BMM-medium. Dissolved oxygen (pO) and pH were measured with a wireless measuring system, cell growth was followed by measurement of the OD. Vertical lines represent methanol feeding points in A and C or start of methanol feed in B and D.<p><b>Copyright information:</b></p><p>Taken from "Improved production of human type II procollagen in the yeast in shake flasks by a wireless-controlled fed-batch system"</p><p>http://www.biomedcentral.com/1472-6750/8/33</p><p>BMC Biotechnology 2008;8():33-33.</p><p>Published online 27 Mar 2008</p><p>PMCID:PMC2315644.</p><p></p>