Crane Fly movie.avi

The movie shows an example of the OI-DIC image of a crane fly spermatocyte during metaphase of meiosis-I, taken with a 100x/1.30 oil immersion objective lens. The three autosomal, bivalent chromosomes are pulled apart at the spindle equator, along with one of the X-Y sex univalents located at the right. The distribution of tubular mitochondria surrounding the spindle and granual chromosome structure is clearly visible. The experiment was done by M. Shribak and J. R. LaFountain, Jr. (University at Buffalo, Buffalo, NY) (Shribak et al., 2008). David Biggs, of KB Imaging Solutions LLC, Loomis, CA, computed the optical path difference (OPD) image, using iterative deconvolution algorithms. Image acquisition and processing took about 1 second each. Image size is 68 µm x68 µm. We took one OPD image each 30 seconds. The overall time frame of this time-lapse video is 30 min.