mp7b00177_si_001.pdf (927.85 kB)
Controlling Structure and Function of Polymeric Drug Delivery Nanoparticles Using Microfluidics
journal contribution
posted on 2017-05-18, 00:00 authored by Aman Bains, Yimeng Cao, Sundiata Kly, Jeremy E. Wulff, Matthew G. MoffittWe demonstrate control of multiscale
structure and drug delivery
function for paclitaxel (PAX)-loaded polycaprolactone-block-poly(ethylene oxide) (PCL-b-PEO) polymeric nanoparticles
(PNPs) via synthesis and flow-directed shear processing in a two-phase
gas–liquid microfluidic reactor. This strategy takes a page
from the engineering of commodity plastics, where processing rather
than polymer chemistry provides an experimental handle on properties
and function. PNPs formed from copolymers with three different PCL
block lengths show sizes, morphologies, and loading efficiencies that
depend on both the PCL block length and the microfluidic flow rate.
By varying flow rate and comparing with a conventional bulk method
of PNP preparation, we show that flow-variable shear processing provides
control of PNP sizes and morphologies and enables slower PAX release
times (up to 2 weeks) compared to bulk-prepared PNPs. Antiproliferative
effects against cultured MCF-7 breast cancer cells were greatest for
PNPs formed at an intermediate flow rate, corresponding to small and
low-polydispersity spheres formed uniquely at this flow condition.
Formation and flow-directed nanoscale shear processing in gas–liquid
microfluidic reactors provides a manufacturing platform for drug delivery
PNPs that could enable more effective and selective nanomedicines
through multiscale structural control.