figshare
Browse
Fig 6.tif (5.8 MB)

Confocal time-lapse analysis of 3xHA-Digitor-mCitrine during mitosis in a syncytial Drosophila embryo.

Download (5.8 MB)
figure
posted on 2016-11-18, 17:59 authored by Saheli Sengupta, Uttama Rath, Changfu Yao, Michael Zavortink, Chao Wang, Jack Girton, Kristen M. Johansen, Jørgen Johansen

The figure shows the relative dynamics of the 3xHA-Digitor-mCitrine construct (in green) and tubulin-mCherry (in red) at interphase and metaphase, respectively. Arrows indicate the localization of the 3xHA-Digitor-mCitrine construct in the spindle region after NEB and microtubule spindle formation at metaphase.

History

Usage metrics

    PLOS ONE

    Categories

    Keywords

    ZNFDrosophila DevelopmentP-element insertionparaquat-induced oxidative stressγ H 2Avspindle matrix protein Skeletoryeast two-hybrid screenMultiple RolesASCIZspindle regionATMINpupal developmentdisc eversionpupal lethalitystress test paradigmDNA double-strand breaksmitotic localizationpuff chromosomal regionsmitotic progressionDNA damage responsespindle matrixmCitrine-labeled Digitorbrain sizePhenotypic analysis

    Licence

    CC BY 4.0

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC