bm5b01040_si_001.pdf (1.48 MB)
Composition and Function of Spider Glues Maintained During the Evolution of Cobwebs
journal contribution
posted on 2015-10-12, 00:00 authored by Dharamdeep Jain, Ci Zhang, Lydia
Rose Cool, Todd A. Blackledge, Chrys Wesdemiotis, Toshikazu Miyoshi, Ali DhinojwalaCapture
silks are an interesting class of biological glues that
help spiders subdue their prey. Viscid capture silk produced by the
orb web spiders is a combination of hygroscopic salts that aid in
water uptake and interact with adhesive glycoproteins to make them
soft and sticky. The orb was a stepping stone to the evolution of
new web types, but little is known about the adhesives in these webs.
For instance, cobweb spiders evolved from orb-weaving ancestors and
utilize glue in specialized sticky gumfoot threads rather than an
elastic spiral. Early investigation suggests that gumfoot adhesives
are quite different viscid glues because they lack a visible glycoprotein
core, act as viscoelastic fluids rather than solids, and are largely
invariant to humidity. Here, we use spectroscopic and staining methods
to show that the gumfoot silk produced by Latrodectus
hesperus (western black widow) is composed of hygroscopic
organic salts and water insoluble glycoproteins, similar to viscid
silk, in addition to a low concentration of spider coating peptides
reported before. Our adhesion studies reveal that the organic salts
play an important role in adhesion, similar to that seen in orb web
spiders, but modulating function at much lower humidity. Our work
shows more similarities in the viscid silk produced by orb web and
cobweb spiders than previously anticipated and provide guidelines
for developing synthetic adhesives that can work in dry to humid environments.