Community portal for the Citrusgreening disease resources

Huanglongbing (HLB) is a tritrophic disease complex involving citrus host trees, the Asian citrus psyllid (ACP) insect and a phloem restricted, bacterial pathogen Candidatus Liberibacter asiaticus (CLas). HLB is considered to be the most devastating of all citrus diseases, and there is currently no adequate control strategy. In Florida, an estimated 40-70% of all citrus trees are infected, and HLB effects include production declines (10-20% per year), diminished fruit quality and increased production costs. We have designed a web portal with information for consumers and growers as well as genomics and bioinformatics resources for citrus, ACP and CLas. The JBrowse browser provides the context for expression data and annotated features on the genome. Biocyc Pathway Tools databases model biochemical pathways within each organism and will be used to explore the entire disease complex. Micro-CT analysis of the ACP will be combined with transcriptomics data from different tissues, life stages and sexes to create a 3D atlas that will reveal the internal anatomy of ACP overlaid with the expression profile of different tissues across major life stages. All tools like JBrowse, Blast and the Atlas will connect to a central database containing gene models for citrus, ACP and multiple Candidatus Liberibacter pathogens. The database will allow manual curation so that the community can continuously improve the knowledgebase as more experimental research is published. The database architecture combines custom schemas and the community developed Chado schema (http://gmod.org/wiki/Chado) for compatibility with other genome databases.