cs4010387_si_001.pdf (1.45 MB)
Combination of Asymmetric Organo- and Biocatalytic Reactions in Organic Media Using Immobilized Catalysts in Different Compartments
journal contribution
posted on 2014-04-04, 00:00 authored by Marcel Heidlindemann, Giuseppe Rulli, Albrecht Berkessel, Werner Hummel, Harald GrögerA proof
of concept for the combination of an asymmetric organocatalytic
reaction with a biotransformation toward a “one-pot like”
process for 1,3-diols based on immobilized organo- and biocatalysts,
which are utilized in different compartments, is demonstrated. This
process which runs completely in organic media consists of an initial
proline-derivative-catalyzed aldol reaction and a subsequent reduction
of the aldol adduct catalyzed by an alcohol dehydrogenase (ADH) without
the need for intermediate isolation. Economically attractive superabsorber-based
coimmobilization for the ADH and its cofactor NAD+ turned
out to give a highly efficient biocatalyst with excellent reusability
and simple product separation from the immobilizate under avoidance
of any tedious extraction steps during the overall process.