jp305597y_si_001.pdf (13.27 kB)
Co-Grafting of Amino–Poly(ethylene glycol) and Magainin I on a TiO2 Surface: Tests of Antifouling and Antibacterial Activities
journal contribution
posted on 2016-02-20, 04:58 authored by Jessie Peyre, Vincent Humblot, Christophe Méthivier, Jean-Marc Berjeaud, Claire-Marie PradierAn antimicrobial peptide, Magainin
I (Mag), was grafted to a titanium oxide surface, via an antiadhesive
poly(ethylene glycol) (PEG) cross-linker. The latter plays a 2-fold
part, being antiadhesive, and enabling the covalent immobilization
of the peptide. The functionalization was characterized at each step
by reflection absorption infrared spectroscopy (RAIRS) and X-ray photoelectron
spectroscopy (XPS). The antiadhesive properties of PEG, and antibacterial
activity of the anchored Magainin I, were individually tested toward
adsorption of bovin serum albumin (BSA) proteins, and against Gram
positive bacteria, Listeria ivanovii, respectively. The results reveal that adhesion of both proteins
and bacteria have been considerably reduced, accompanied by an inhibition
of the growth of remaining adhered bacteria. This work thus offers
a novel approach to functionalize oxide surfaces against biofilms
and to measure the so-obtained properties in each of the successive
steps of a biofilm formation.