Cluster analysis of gene expression of P. aeruginosa genes in response to water.
The PAO1 mini-Tn5-luxCDABE mutant library containing 1369 transcriptional lux fusion strains was inoculated into water in black 96 well microplates and incubated at room temperature. At each time point the optical density (OD600) and luminescence (counts per second) was measured. Gene expression (CPS) readings were taken at day 0, 0.3, 1, 3, 5, 7, 13, 20, 26, and 34. Luminescence was divided by absorbance and fold changes were calculated based on the change in expression (CPS/OD600) compared to time 0. Cluster analysis was performed using Tree View and Cluster 3.0 software. Orange indicates repression, and blue indicates induced expression, relative to the time zero point. Genes with no change in expression are in white. Black bars highlight clusters of genes that are induced late (A, D), throughout (B) or early (C) in the 34 day time period of incubation in water.
