figshare
Browse
1/1
3 files

Circadian PER2 protein oscillations do not persist in cycloheximide-treated mouse embryonic fibroblasts in culture

dataset
posted on 2017-12-28, 16:03 authored by Motomi Tainaka, Masao Doi, Yuichi Inoue, Iori Murai, Hitoshi Okamura

It is not known whether the endogenous mammalian core clock proteins sustain measurable oscillations in cells in culture where de novo translation is pharmacologically inhibited. We studied here the mammalian core clock protein PER2, which undergoes robust circadian oscillations in both abundance and phosphorylation. With a newly developed antibody that enables tracing the endogenous PER2 protein oscillations over circadian cycles with cultured mouse embryonic fibroblast cells, we provide evidence that PER2 does not persist noticeable circadian rhythms when translation is inhibited.

History

Usage metrics

    Chronobiology International

    Licence

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC