Chemical profiling of the tuber of <i>Stephania cambodica</i> Gagnep. (Menispermaceae) and analytical control by UHPLC-DAD

<p>A new aporphine glycoside (<b>1</b>), named ‘angkorwatine’, and eight known alkaloids: oblongine (<b>2</b>), stepharine (<b>3</b>), asimilobine-β-d-glucopyranoside (<b>4</b>), isocorydine (<b>5</b>), tetrahydropalmatine (THP) (<b>6</b>), jatrorrhizine (<b>7</b>), palmatine (PAL) (<b>8</b>), and roemerine (ROE) (<b>9</b>) were simultaneously isolated from the tuber of <i>Stephania cambodica</i>. The development and validation of UHPLC-DAD method was carried out for the quantification of marker compounds (PAL, ROE, THP) of <i>S. cambodica</i>. In addition to good selectivity and linearity (<i>r</i><sup>2</sup> > 0.997), trueness, precision, and accuracy of the method did not exceed the acceptance limit of ±10% for ROE, THP and ±20% for PAL. Consequently, this method is able to provide accurate results between 1.39–4.18 μg/mL, 2.01–30.72 μg/mL, and 4.29–64.42 μg/mL for PAL, ROE, and THP, respectively. This study shows that the validated UHPLC method is a rapid, innovative and effective analytical approach to control quality of tubers of <i>S. cambodica</i> and to regulate the usage of this plant in traditional medicine.</p>