Chemical composition, antioxidant activities and protein profiling of different parts of Allamanda cathartica

The phytochemical screening and protein profiling of Allamanda cathartica was performed. Biochemical analysis revealed that peroxidase (8730 ± 307 units/g), superoxide dismutase (181 ± 3.79 units/g), catalase (529 ± 28.9 units/g), protease (3598 ± 79.8 units/g), total phenolic contents (19,344 ± 657 μM/g), β-esterases (342 ± 46.5 units/g) and the total oxidant status were highest in the roots as compared to other plant parts. However, total soluble proteins (128 ± 1.54 mg/g), lycopene (5.70 ± 0.61 mg/g), chlorophyll a (161 ± 24.9 μg/g), total chlorophyll content (267 ± 34.3 μg/g) and total carotenoid content (12.4 ± 1.71 mg/g) were found to be highest in leaves. Moreover, total antioxidant capacity (5.43 ± 0.29 μM/g) detected by using ABTS method and α-esterase (714.580 ± 23.6 units/g) were highest in shoots. The protein profiling was performed using SDS–PAGE. In leaves, 13 peptides with molecular weight (M.wt.) from 27 to 168 kDa were detected while in shoots 10 peptides with M.wt. from 30 to 95 kDa were resolved. Similarly, in roots, 10 peptides of 30–880 kDa and in flower seven peptides of 30–88 kDa were detected.