Chemical characterization of Endomorphine-TAMRA conjugates.

<p><b>(A)</b> Chemical structure of EM-TAMRA indicated by modified Endomorphine-1 peptide in blue and TAMRA-Maleimide in magenta. Absorbance and Emission spectra of <b>(B)</b> 200 μM TAMRA-Maleimide and <b>(C)</b> 20 μM EM-TAMRA at pH 7.24. <b>(D)</b> Absorbance of 20 μM EM-TAMRA at different pH 2.15–10. I<sub>Normalized</sub> = Normalized intensity; I<sub>abs</sub> = Normalized absorbance.</p>