ol6b03056_si_001.pdf (6.99 MB)
Chemical Synthesis of HMGA1a Proteins with Post-translational Modifications via Ser/Thr Ligation
journal contribution
posted on 2016-11-04, 18:05 authored by Tianlu Li, Heng Liu, Xuechen LiThe first chemical synthesis of nuclear
protein HMGA1a via Ser/Thr
ligation is reported. Notably, Hmb (2-hydroxy-4-methoxybenzyl) exhibits crucial
improvement of both the difficult coupling during solid phase peptide
synthesis and the poor ligation encountered in protein synthesis.
These efforts led to preparation of HMGA1a analogs with well-defined
phosphorylation and methylation patterns (9 synthetic proteins in
total), thus overcoming the heterogeneous and combinatory problems
inherent to protein post-translational modifications (PTMs), and facilitating
the study of the regulatory roles of such PTMs.