Characterization of lectin affinity-purified 16055 glycan-deleted trimers with the 332 N-glycan restored.

(a) SEC profiles and EM 2D class averages. A1 or B1, A2, A3 and AB indicate trimers with one, two, three and four N-glycan deletions, respectively. SEC profiles of N-glycan-deleted trimers (solid line) are shown in comparison with the +N332 PT trimer (dotted line) and the expression level relative to expression level of +N332 PT is shown on each SEC graph. Percentage of native-like trimers is indicated above the 2D class averages representative images. (b) DSC thermal transition curves and derived T_ms of glycan-deleted trimers (red solid line) compared to the backbone glycoprotein +N332 PT (black dotted line).