ppat.1006614.g002.tif (474.15 kB)
Characterization of lectin affinity-purified 16055 glycan-deleted trimers with the 332 N-glycan restored.
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posted on 2017-09-13, 17:48 authored by Viktoriya Dubrovskaya, Javier Guenaga, Natalia de Val, Richard Wilson, Yu Feng, Arlette Movsesyan, Gunilla B. Karlsson Hedestam, Andrew B. Ward, Richard T. Wyatt(a) SEC profiles and EM 2D class averages. A1 or B1, A2, A3 and AB indicate trimers with one, two, three and four N-glycan deletions, respectively. SEC profiles of N-glycan-deleted trimers (solid line) are shown in comparison with the +N332 PT trimer (dotted line) and the expression level relative to expression level of +N332 PT is shown on each SEC graph. Percentage of native-like trimers is indicated above the 2D class averages representative images. (b) DSC thermal transition curves and derived Tms of glycan-deleted trimers (red solid line) compared to the backbone glycoprotein +N332 PT (black dotted line).
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16055 PNGS-deleted trimersHIV Env NFL trimer integrityCD 4 receptor engagementCD 4bsïve B cellswell-ordered clade C 16055CD 4bs bNAbsB cell repertoireresidue N 276rabbit immunogenicity experimentsB cell responsesantibodyglycosylated NFL trimersCD 4 binding siteglycosylated autologous virustier 2 virusesCD 4bs viruses4 PNGS sitesTargeted N-glycan deletionneutralizing determinantHIV envelope glycoproteinsN-glycan-deleted trimer variantsglycan-deleted trimer variantsB cell recognition
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