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Characterization of cis-4-hydroxy-D-proline dehydrogenase from Sinorhizobium meliloti

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posted on 2017-12-01, 08:58 authored by Seiya Watanabe, Daichi Morimoto, Fumiyasu Fukumori, Yasuo Watanabe

The hypO gene from Sinorhizobium meliloti, located within the trans-4-hydroxy-L-proline metabolic gene cluster, was first successfully expressed in the host Pseudomonas putida. Purified HypO protein functioned as a FAD-containing cis-4-hydroxy-D-proline dehydrogenase with a homomeric structure. In contrast to other known enzymes, significant activity for D-proline was found, confirming a previously proposed potential involvement in D-proline metabolism.

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