Characterization of <i>C</i>. <i>difficile</i> R20291::<i>sinR’</i>.

<p><b>(A)</b><i>C</i>. <i>difficile</i> cultures were grown in 70:30 medium for 30 h under anaerobic conditions and Sporulation frequency (CFU/ml of ethanol resistant spores) of R20291, <i>sinR’</i> mutant was determined. The data shown are means ± standard errors of three biological replicates. <b>(B)</b> Motility assays of the <i>C</i>. <i>difficile</i> R20291, <i>sinR’</i> mutant and complemented <i>sinR’</i> mutant. The experiments were repeated at least three times independently (*, <i>P</i>≤0.05 by a two-tailed Student's <i>t</i>-test). <b>(C)</b> Toxin production measured by ELISA. Statistical analysis was performed using one way-ANOVA with Dunnett’s multiple comparisons test comparing values to the average of the parent with vector control (***<0.0005, *< 0.05 <i>p</i>-value).</p>