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Characterization of Synergistic Embryotoxicity of Nickel and Buprofezin in Zebrafish
journal contribution
posted on 2015-04-07, 00:00 authored by Tingting Ku, Wei Yan, Wuyao Jia, Yang Yun, Na Zhu, Guangke Li, Nan SangMultiple
pollutants, usually at low levels, coexist and may interact
in the environment. It is therefore important to analyze the toxicity
of mixtures of coexisting chemicals to evaluate the potential ecological
risk. Concern regarding the co-occurrence and combined bioeffects
of heavy metals and organic insecticides in aquatic settings has existed
for many years, but a clear understanding of the interactions between
and potential combined toxicity of these chemicals remains elusive.
In the present study, the combined effects of the heavy metal nickel
(NiSO4) and insect growth regulator buprofezin on the induction
of embryo toxicity in zebrafish were assessed. By applying nonlinear
regression to the concentration–response data with each of
the chemicals using the Hill and Langmuir functions and computing
the predictions using the model of concentration addition (CA), we
confirmed that NiSO4 and buprofezin acted together to produce
synergistic embryotoxicity in zebrafish. Subsequently, we further
found that the combination of NiSO4 and buprofezin formed
a complex that facilitated the uptake of nickel (Ni) and buprofezin
by the embryos. Following this, we clarified that an oxidative mechanism
of the complex might underlie the synergistic embryotoxicity of NiSO4 and buprofezin.