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Characterization of Genes for a Putative Hydroxycinnamoyl-coenzyme A Quinate Transferase and p‑Coumarate 3′-Hydroxylase and Chlorogenic Acid Accumulation in Tartary Buckwheat
journal contribution
posted on 2013-05-01, 00:00 authored by Yeon Bok Kim, Aye Aye Thwe, Ye Ji Kim, Xiaohua Li, Haeng Hoon Kim, Phun Bum Park, Tastsuro Suzuki, Sun-Ju Kim, Sang Un ParkTartary buckwheat (Fagopyrum tataricum Gaertn.) contains a high level
of flavonoid compounds, which have
beneficial and pharmacological effects on health. In this study, we
isolated full-length cDNAs encoding hydroxycinnamoyl-coenzyme A quinate
hydroxycinnamoyltransferase (HQT) and p-coumarate
3′-hydroxylase (C3H), which are involved in chlorogenic acid
(CGA) biosynthesis. We examined the expression levels of HQT and C3H
using real-time RT-PCR in different organs and sprouts of two tartary
buckwheat cultivars (Hokkai T8 and T10) and analyzed CGA content using
high-performance liquid chromatography. Among the organs, the flowers
in both cultivars showed the highest levels of CGA. We concluded that
the expression pattern of FtHQT and FtC3H did not match the accumulation pattern of CGA in different organs
of T8 and T10 cultivars. Gene expression and CGA content varied between
the cultivars. We presume that FtHQT and FtC3H levels might be controlled by multiple metabolic pathways
in different organs of tartary buckwheat. Probably, FtC3H might have a greater effect on CGA biosynthesis than FtHQT. Our results will be helpful for a greater understanding of CGA
biosynthesis in tartary buckwheat.