la204863s_si_001.pdf (2.48 MB)
Cellular Microfabrication: Observing Intercellular Interactions Using Lithographically-Defined DNA Capture Sequences
journal contribution
posted on 2016-02-20, 20:50 authored by Hiroaki Onoe, Sonny C. Hsiao, Erik S. Douglas, Zev J. Gartner, Carolyn
R. Bertozzi, Matthew
B. Francis, Richard A. MathiesPrevious reports have shown that synthetic DNA strands
can be attached
to the plasma membrane of living cells to equip them with artificial
adhesion “receptors” that bind to complementary strands
extending from material surfaces. This approach is compatible with
a wide range of cell types, offers excellent capture efficiency, and
can potentially be used to create complex multicellular arrangements
through the use of multiple capture sequences. In this work, we apply
an aluminum “lift off” lithography method to allow the
efficient generation of complex patterns comprising different DNA
sequences. The resulting surfaces are then demonstrated to be able
to capture up to three distinct types of living cells in specific
locations. The utility of this approach is demonstrated through the
observation of patterned cells as they communicate by diffusion-based
paracrine signaling. It is anticipated that the ability of this technique
to create virtually any type of 2D heterogeneous cell pattern should
prove highly useful for the examination of key questions in cell signaling,
including stem cell differentiation and cancer metastasis.