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Cell viability during 4–8 h of cyclic stretch.

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posted on 2017-09-12, 17:41 authored by Jing Chang, Yuefeng Xia, Karla Wasserloos, Meihong Deng, Kory J. Blose, David A. Vorp, Heth R. Turnquist, Timothy R. Billiar, Bruce A. Pitt, Ma-Zhong Zhang, Li-Ming Zhang

MLE-12 cells were conditioned in serum free medium for 12 h and then transferred to wells in Bioflex and underwent cyclic stretch (cyclic S) or were not stretch (con) for 4, 6 or 8 h. Viable cells were negative for Annexin-V and propidium iodide (PI) staining (Q3), early-stage apoptotic cells were positive for Annexin-V staining, but negative for PI staining (Q4), and late-stage apoptotic cells were positive for Annexin-V and PI staining (Q2).

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