Cell proliferation assays for BAECs exposed to elevated hydrostatic pressure for indicated periods and then subjected to rapid depressurization or slow depressurization along with a negative control (atmospheric pressure).

(A) CyQUANT assay results after 24 hr application of elevated hydrostatic pressure as a function of initial cell seeding density within single wells of a 96 multiwell plate. Data represents mean +/-SE for 3–5 replicates within each experimental group. (B) Cell counts within 6 well plates taken using a hemocytometer as a function of days exposed to elevated hydrostatic pressure. Data displayed is an average of two individual experimental runs (exception is the 9 day cell count point for the atmospheric pressure negative control data set; this represents a single run). Two to four cell counts were taken at each time for each run. Error bars represent standard error.