Fig 1.tif (523.74 kB)
Cartoon showing the influence of the Subtract method into the standard samples.
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posted on 2017-11-14, 18:41 authored by Hector Sanz, John J. Aponte, Jaroslaw Harezlak, Yan Dong, Aintzane Ayestaran, Augusto Nhabomba, Maxmillian Mpina, Obiang Régis Maurin, Núria Díez-Padrisa, Ruth Aguilar, Gemma Moncunill, Agnandij Selidji Todagbe, Claudia Daubenberger, Carlota Dobaño, Clarissa ValimBackground noise calculated as the geometric mean of the blank controls is shown as a horizontal dashed line. (A) Standard curve plotting the MFI in the original scale as a function of concentration; (B) Standard curve plotting the MFI in the original scale as a function of concentration after subtracting the MFI of the blank control from all standard samples; (C) Standard curve plotting the MFI in the log10 scale as a function of concentration; and (D) Standard curve plotting the MFI in the log10 scale as a function of concentration after subtracting the MFI of the blank control from all standard samples.
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quality controlSuboptimal calibrationfitnessRTSmethodmalariavaccinemultiplex bead-based immunoassays data analysis Multiplex bead-based immunoassaysbackground noisemultiplex bead-based immunoassayestimationfunctionSCPhase 3 randomizedsamplequantificationanalyteconcentrationdrLumiconduct quality control
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