Legler's Stream Frog (Ptychohyla legleri) is an uncommon and imperiled treefrog distributed along the Pacific versant of southern Costa Rica and western Panama. We observed novel calling behavior of male P. legleri, where individuals were calling diurnally in duets while being concealed in terrestrial perches within caves. Here we provide video and audio files demonstrating these behaviors. We suggests further research investigate the consequences of microhabitat site selection for P. legleri; perhaps males call from subterranean perches because they provide quality oviposition sites.