CTB binding to primary human intestinal cells can be blocked by interference with fucosylated structures.
2018-02-12T18:46:33Z (GMT) by
<p><b>(A)</b> Bar graph showing relative absorbance values from an ELISA with immobilized anti-Le<sup>X</sup>, and detection with CTB-HRP. Samples as indicated from lysates of isolated human cells (2 μg protein/ml). Each dot represents a human donor (n = 5–8). <b>(B)</b> CD66 or <b>(C)</b> CD66 and Le<sup>X</sup> expression by jejunal epithelial cells that were isolated using EDTA medium (villi) or enzymatic degradation after EDTA treatment (non-villi or crypt). Histograms from flow cytometry analyses of CTB-, G33D- and OVA-binding to the differentially enriched epithelial cells. <b>(B)</b> EpCAM<sup>+</sup> cells and <b>(C)</b> EpCAM<sup>+</sup>Le<sup>X+</sup> cells. <b>(D-G)</b> Bar graph showing percent of gMFI of CTB binding to jejunal epithelial cells by pretreatment of the cells with <b>(D)</b> lectins, <b>(E)</b> sugars, <b>(F)</b> oligosaccharides and <b>(G)</b> HSA-linked oligosaccharides. Graphs show the percent of gMFI of CTB binding to the cells where 100% represents CTB staining with no blocking oligosaccharide. Each dot represents a donor in <b>(D)</b> n = 4–12, <b>(E)</b> n = 6–8, <b>(F)</b> n = 6–12, <b>(G)</b> n = 6–7. Significance was calculated using a one-way-ANOVA with Tukey correction compared to CTB without block if not indicated otherwise with bars (**** = p<0,0001, *** = p<0,005, ** = p<0,01 and * = p<0,05).</p>
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