Bubble imaging and TEWL measures with evaporimetry in two subjects (EB-02, 03) who were not stimulated.

<p>(A-B) show sweat bubble imaging after 20 min with no applied stimulus (and with no atropine to block any baseline cholinergic secretion). (C-D) show evaporimetry traces at the same sites. The dashed line shows a stable and consistent difference between the two subjects of ~6 g/(m<sup>2</sup>·hr) or 10 nl/(cm<sup>2</sup>·min). The summed bubble secretion corrected for the 0.6 cm<sup>2</sup> size of the imaged area was 13.4 nl/cm<sup>2</sup> ·min. This agreement is at least as good as the agreement for bubble tests at same sites on different days shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0165254#pone.0165254.g005" target="_blank">Fig 5</a>. However, in subsequent tests with this subject no bubbles were observed in spite of the same TEWL (see text). (E) Distribution of single gland basal secretion rates for sweat bubbles (n = 66) shown in panel (B). Each point plots the volume (y-axis) and rank for an individual gland.</p>