np7b00707_si_001.pdf (1.18 MB)
Bioactivity-Guided Isolation of Potential Antidiabetic and Antihyperlipidemic Compounds from Trigonella stellata
journal contribution
posted on 2018-04-20, 18:37 authored by Safa M. Shams Eldin, Mohamed
M. Radwan, Amira S. Wanas, Abdel-Azim M. Habib, Fahima F. Kassem, Hala M. Hammoda, Shabana
I. Khan, Michael L. Klein, Khaled M. Elokely, Mahmoud A. ElSohlyThe in vitro antidiabetic and antihyperlipidemic
activities of
an alcoholic extract of Trigonella stellata were
evaluated in terms of the activation of PPARα and
PPARγ in human hepatoma (HepG2) cells. The extract
was investigated phytochemically, aiming at the isolation of the most
active compounds to be used as a platform for drug discovery. Three
new isoflavans, (3S,4R)-4,2′,4′-trihydroxy)-7-methoxyisoflavan
(1), (3R,4S)-4,2′,4′-trihydroxy-7-methoxy-4′-O-β-d-glucopyranosylisoflavan (2), and (2S,3R,4R)-4,2′,4′-trihydroxy-2,7-dimethoxyisoflavan (3), were isolated and characterized along with the five known
compounds p-hydroxybenzoic acid (4),
7,4′-dihydroxyflavone (5), dihydromelilotoside
(6), quercetin-3,7-O-α-l-dirhamnoside (7), and soyasaponin I (8). The structures of 1–3 were elucidated
using various spectroscopic techniques including HRESIMS and 1D and
2D NMR. The absolute stereochemistry of the new isoflavans (1–3) was determined using both experimental
and calculated electronic circular dichroism as well as DP4 calculations.
The isolated compounds were tested for their PPARα and PPARγ activation effects in HepG2 cells.