(Further detailed explanation provided inside the archive)
RPE dataset for the article:
- L. Nanni, M. Paci, F. L. C. Santos, H. Skottman, K. Juuti-Uusitalo, J. Hyttinen, Texture descriptors ensembles enable image-based classification of maturation of human stem cell-derived retinal pigmented epithelium, Plos One 2016
Image acquisition
Each RPE cell culture was imaged with the same settings. The microscope used was a Nikon Eclipse TE200S phase-contrast microscope (Nikon Instruments Europe B.V., Amstelveen, Netherlands) with the 20x objective and Ph1 phase contrast.
Cell imaging parameters are the following:
- Quality 2560 x 1920 pixels
- Mode Manual exposure
- Exposure 25 – 125 ms
- AE compensation 0
- Gain 1.2 x
- Contrast Dynamic
Subwindows dividing process
The dataset is composed of 195 images that were divided into 16 subwindows and each subwindow labeled into 4 classes by two trained operators. Every subwindow that presented clutters, out of focus elements or just background was discarded and is not part of this dataset.
Subwindows coding
Each subwindow was renamed after manual classification using a specific code. This code was assembled by five elements: