Binding of FGF2-Luc with the endogenous FGF receptor.

<p>Untransfected HEK293T cells were used as the receptor source. <b>(A)</b> Saturation binding of FGF2-Luc with the endogenous FGF receptor. Nonspecific binding data were obtained by competition with 250 nM of 6×His-FGF2. The measured bioluminescence data were expressed as mean ± SE (<i>n</i> = 3). The total binding data were fitted to Y = B<sub>max</sub>X/(K<sub>d</sub> + X) + N<sub>s</sub>X, specific binding data to Y = B<sub>max</sub>X/(K<sub>d</sub> + X), and nonspecific binding data to a linear curve. <b>Inner panel,</b> Scatchard plot of the specific binding data. <b>(B)</b> Competition binding of wild-type or mutant FGF2s with the endogenous FGF receptor using FGF2-Luc as a tracer. The measured bioluminescence data were expressed as mean ± SE (<i>n</i> = 3) and fitted with sigmoidal curves using the SigmaPlot10.0 software.</p>