BPNT1 depletion induces XRN2 de-repression.

<p>(A) <i>rpl-43</i><sub><i>Prom</i></sub>::<i>rpl-43</i><sub><i>Body</i></sub>::<i>rpl-43</i><sub><i>ICR</i></sub> reporter animals in wild-type (<i>bpnt-1(+)</i>) or <i>bpnt-1(xe22)</i> genetic background were cultured from L1 to L4 for 40 hours at 20°C and observed. GFP signal was detected in hypodermal (top), intestinal (middle, arrows) and vulval (bottom) cells. Positions of vulvae are indicated by square brackets. Corresponding DIC images of mid-L4 stage vulvae are shown (bottom). Scale bar: 100 μm. (B) Diagram of an inferred BPNT1-XRN2 regulatory network. (C) <i>rpl-43</i><sub><i>Prom</i></sub>::<i>rpl-43</i><sub><i>Body</i></sub>::<i>rpl-43</i><sub><i>ICR</i></sub> reporter animals were exposed to mock or <i>xrn-2</i> RNAi from L1 to L4 at 20°C and observed. GFP signal was detected in hypodermal (top), intestinal (middle, arrows) and vulval (bottom) cells. Images are shown as described in (A).</p>