Axonal interferon treatment does not alter retrograde axonal transport of RABV particles.

(A) Live particle tracking setup (see also S3 Fig). IFNβ or IFNγ was added to N for 24 h prior to infection in N. (B) Motile RABV particle tracks (black lines) visualized by maximum intensity projections from each field of view (FOV) along the M compartment barrier at 2–4 hpi in N (scale bars = 20 μm). One FOV is shown per condition. Blue boxes highlight tracks of particles that moved retrograde for the entire duration of the 15 sec movie (163 frames). (C) For tracks in blue boxes, kymographs show the displacement of each particle over time. Diagonal lines indicate particles moving retrograde. (D) Quantification of the number of RABV particles moving retrograde per FOV (+/-) IFNβ or IFNγ pretreatment in N. Each open circle represents an individual FOV. Vertical lines and error bars represent the mean ± SD for each condition (ns = not significant using one-way ANOVA). Total FOVs counted were 308 (no treatment), 297 (IFNβ), and 309 (IFNγ) across 3 independent replicate chambers per condition.