Assessment of phytochemicals, antioxidant and antimutagenic activity in micropropagated plants of <i>Quercus serrata,</i> a high value tree species of Himalaya

<p>The major aim of the present study was to: (i) develop an efficient clonal <i>in vitro</i> propagation procedure, and (ii) investigate and compare phytochemicals, antioxidant, and DNA damage protection activity in methanolic extracts of <i>in vitro</i> raised and mother plant leaves of <i>Quercus serrata</i>. The study succeeded in establishing an efficient clonal micropropagation procedure using nodal segments taken from <i>in vivo</i> growing mother plant. The WP medium supplemented with six-benzyleaminopurine (BAP: 8.88 μM) plus indole-3-acetic acid (IAA: 1.43μM) produced the highest number of shoots/explant having maximum average shoot length. <i>In vitro</i> derived shoots exhibited best rooting upon application of 100μM indole-3-butyric acid for 48 h. Among studied phytochemicals, the total phenol content was recorded higher from <i>in vitro</i> raised plants and total flavonoid in the mother plant. Antioxidant activities were recorded higher in case of <i>in vitro</i> raised plants. Also, the methanolic leaf extract of <i>in vitro</i> raised plants exhibited higher antimutagenic activity that of mother plant. These findings indicate <i>Q. serrata</i> as a promising source of natural antioxidants that could be utilized commercially for the prevention of photoaging and oxidative stress-mediated skin diseases.</p>