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Application of the proposed method to the EFTEM N-maps.

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posted on 2018-12-11, 18:43 authored by Tatiana Ismagulova, Anastasia Shebanova, Olga Gorelova, Olga Baulina, Alexei Solovchenko

(A-E) The results of the method application to a cyanobacterium Nostoc sp. PCC 7118 from the P-starved culture. (F-J) The results of the method application to a eukaryotic microalga Chlorella vulgaris IPPAS C-1 from the P-starved culture. (A) and (F) Elastically filtered TEM images of cell sections. (B) and (G) EFTEM N-maps of the cell sections. (D) and (I) Averaged profiles of the EFTEM N-maps. (C) and (H) The EFTEM maps processed according to the workflow “A” (see text and Fig 1). (E) and (J) The relative entropy analysis of the EFTEM maps (B) and (G), respectively. The averaged profiles were recorded along the white lines (see the N-maps). The red outline on the processed maps (C) and (H) indicates the region taken for the inclusion area measurements. In the graphs (E) and (J) the threshold pixels g0 used for the EFTEM maps processing are designated (for details see text). CG cyanophycin granule, T thylakoid(s), β high electron density lipid β-granules, Ch chloroplast, NRI nitrogen-rich inclusion, S starch, P pyrenoid, OB oil body. Scale bars = 0.5 μm.

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