Gni099f3.jpg (28.72 kB)
Apparent turnover rate of E9 DNase with 17mer ssDNA substrate
figure
posted on 2011-12-30, 15:04 authored by Robert H. H. van den Heuvel, Sara Gato, Cees Versluis, Pascal Gerbaux, Colin Kleanthous, Albert J. R. HeckCopyright information:
Taken from "Real-time monitoring of enzymatic DNA hydrolysis by electrospray ionization mass spectrometry"
Nucleic Acids Research 2005;33(10):e96-e96.
Published online 13 Jun 2005
PMCID:PMC1150282.
© The Author 2005. Published by Oxford University Press. All rights reserved
Progress curve of reaction catalyzed by E9 DNase (10 μ) in the presence of Ni (20 μ) and 17mer ssDNA (800 μ) at 20°C. ssDNA 17mer, fragment and fragment are indicated by triangles, squares and diamonds, respectively. The calculated turnover rate is 0.71 ± 0.10 s.