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Antigen-Specific Monoclonal Antibodies Isolated from B Cells Expressing Constitutively Active STAT5

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posted on 2011-04-15, 02:05 authored by Ferenc A. Scheeren, Caroline M. M. van Geelen, Etsuko Yasuda, Hergen Spits, Tim Beaumont

Background

Fully human monoclonal antibodies directed against specific pathogens have a high therapeutic potential, but are difficult to generate.

Methodology/Principal Findings

Memory B cells were immortalized by expressing an inducible active mutant of the transcription factor Signal Transducer and Activator of Transcription 5 (STAT5). Active STAT5 inhibits the differentiation of B cells while increasing their replicative life span. We obtained cloned B cell lines, which produced antibodies in the presence of interleukin 21 after turning off STAT5. We used this method to obtain monoclonal antibodies against the model antigen tetanus toxin.

Conclusions/Significance

Here we describe a novel and relatively simple method of immortalizing antigen-specific human B cells for isolation of human monoclonal antibodies. These results show that STAT5 overexpression can be employed to isolate antigen specific antibodies from human memory B cells.

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