es6b05100_si_001.pdf (391.7 kB)
Antibiotic Resistance Genes and Correlations with Microbial Community and Metal Resistance Genes in Full-Scale Biogas Reactors As Revealed by Metagenomic Analysis
journal contribution
posted on 2017-03-08, 00:00 authored by Gang Luo, Bing Li, Li-Guan Li, Tong Zhang, Irini AngelidakiDigested
residues from biogas plants are often used as biofertilizers
for agricultural crops cultivation. The antibiotic resistance genes
(ARGs) in digested residues pose a high risk to public health due
to their potential spread to the disease-causing microorganisms and
thus reduce the susceptibility of disease-causing microorganisms to
antibiotics in medical treatment. A high-throughput sequencing (HTS)-based
metagenomic approach was used in the present study to investigate
the variations of ARGs in full-scale biogas reactors and the correlations
of ARGs with microbial communities and metal resistance genes (MRGs).
The total abundance of ARGs in all the samples varied from 7 ×
10–3 to 1.08 × 10–1 copy
of ARG/copy of 16S-rRNA gene, and the samples obtained from thermophilic
biogas reactors had a lower total abundance of ARGs, indicating the
superiority of thermophilic anaerobic digestion for ARGs removal.
ARGs in all the samples were composed of 175 ARG subtypes; however,
only 7 ARG subtypes were shared by all the samples. Principal component
analysis and canonical correspondence analysis clustered the samples
into three groups (samples from manure-based mesophilic reactors,
manure-based thermophilic reactors, and sludge-based mesophilic reactors),
and substrate, temperature, and hydraulic retention time (HRT) as
well as volatile fatty acids (VFAs) were identified as crucial environmental
variables affecting the ARGs compositions. Procrustes analysis revealed
microbial community composition was the determinant of ARGs composition
in biogas reactors, and there was also a significant correlation between
ARGs composition and MRGs composition. Network analysis further revealed
the co-occurrence of ARGs with specific microorganisms and MRGs.
History
Usage metrics
Categories
Keywords
VFA16 S-rRNA genemanure-based mesophilic reactorsHRTMRGARGs compositionAntibiotic Resistance Genes7 ARG subtypesHTSFull-Scale Biogas ReactorsPrincipal component analysisMetal Resistance Genessamplemetal resistance genesdisease-causing microorganismsantibiotic resistance genesmanure-based thermophilic reactors175 ARG subtypesthermophilic biogas reactorsbiogas reactorscanonical correspondence analysisMetagenomic Analysis Digested residues
Licence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC