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Anti-staphylococcal Activity of Injectable Nano Tigecycline/Chitosan-PRP Composite Hydrogel Using Drosophila melanogaster Model for Infectious Wounds
journal contribution
posted on 2016-08-10, 00:00 authored by T. R. Nimal, Gaurav Baranwal, M. C. Bavya, Raja Biswas, R. JayakumarCompared to the current treatment
modalities, the use of an injectable hydrogel system, loaded with
antibiotic encapsulated nanoparticles for the purpose of treating Staphylococcus aureus (S. aureus) chronic
wound infections have several advantages. These include adhesiveness
to infection site, reduced frequency of dressings, sustained drug
release, inhibition of bacterial growth, and increased healing. In
the present work tigecycline nanoparticles were loaded into chitosan–platelet-rich
plasma (PRP) hydrogel. The tigecycline nanoparticles (95 ± 13
nm) were synthesized through ionic cross-linking method using chitosan,
tripolyphosphate, and tigecycline and characterized by dynamic light
scattering (DLS), scanning electron microscope (SEM), and Fourier
transform infrared spectroscopy (FT-IR). The synthesized nanoparticles
and activated PRP powder were mixed with chitosan hydrogel to form
a homogeneous gel. Rheology studies have confirmed the shear thinning
property, thermal stability, and injectability of the prepared gel
systems. The gel system was further assessed for its drug release
property and found that it was released in a sustained manner. Hemolysis
and blood-clotting assays demonstrated that the gel system was neither
a hemolysin nor a hamper to the clotting cascade. Cell viability results
showed that these nanoparticles were cyto-compatible. The bioactivity
of PRP loaded chitosan gel toward fibroblast cell line was studied
using cell proliferation and migration assay. In vitro antibacterial studies revealed that the gel system inhibited bacterial
growth to a great extent. The antibacterial activity was further analyzed
using ex vivo porcine skin assay. In vivo anti-Staphylococcal activity of the prepared hydrogels was studied
using a Drosophila melanogaster infection model.
The tigecycline and tigecycline nanoparticle incorporated chitosan
gel showed a significant antibacterial activity against S.
aureus. Thus, the gel system is an effective medium for antibiotic
delivery and can be applied on the infection sites to effectively
forestall various skin infections caused by S. aureus.