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Anti-Blomia tropicalis IgE levels in serum samples assayed using as antigens B. tropicalis extract depleted or not of carbohydrate.
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posted on 2017-03-28, 18:21 authored by Neuza M. Alcantara-Neves, Rafael V. Veiga, João C. M. Ponte, Sérgio S. da Cunha, Silvia M. Simões, Álvaro A. Cruz, Maria Yazdanbakhsh, Sheila M. Matos, Thiago Magalhães Silva, Camila A. Figueiredo, Lain C. Pontes-de-Carvalho, Laura C. Rodrigues, Rosemeire L. Fiaccone, Philip J. Cooper, Maurício L. BarretoAnti-Blomia tropicalis IgE levels were measured by indirect in-house ELISA in individuals positive for this antibody in IMMUNOCAP and were divided in two groups: SPT negative or positive for the same allergen. Antigen was treated with methaperiodate and the sera were assayed against raw and methaperiodate treated antigens. *Difference of sIgE results between SPT positive and SPT negative children (Mann–Whitney test). **Difference of sIgE results before and after antigen treatment with methaperiodate (Wilcoxon test).
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skin test reactivityTrichuris trichiura infectionsdissociationSPTIllumina Omni 2.5. ResultsfactorSNPBlatella germanica allergensherpes simplex virusskin prick testingpresenceskin prick test reactivityallergen skin test reactivityELISAskin prick testsBrazilian children BackgroundIgE antibodiestropicaliIGHE
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