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And other similar tRNA genes

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posted on 2011-12-30, 15:04 authored by Shinya Murakami, Katrin Kuehnle, David B. Stern

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Taken from "A spontaneous tRNA suppressor of a mutation in the nuclear gene required for stability of the chloroplast mRNA"

Nucleic Acids Research 2005;33(10):3372-3380.

Published online 9 Jun 2005

PMCID:PMC1148173.

© The Author 2005. Published by Oxford University Press. All rights reserved

() Scaffold 35, which includes , derived from the genome portal v2.0. The positions of and the chromosomal marker (vertical brown lines) are labeled. The five tRNA genes present in the region are shown as vertical brown lines in the lower part of the diagram, and is labeled. Red areas are unsequenced regions, whose sizes are estimated. () Alignment of selected tRNA genes. The genes shown are highly similar to , and needed to be taken into consideration in designing a primer extension expression experiment. Anticodons are boxed, and asterisks indicate positions where a base difference exists in at least one gene. The lower two lines compare the WT and mutant sequences, with the point mutation shown in bold. The horizontal arrow represents the primer used for the assay shown in and described in the text.

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