Analysis of the silent copies recombining into the HV of

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Taken from "A real-time semi-quantitative RT–PCR assay demonstrates that the sequence dictates the frequency and characteristics of pilin antigenic variation in "

Nucleic Acids Research 2005;33(10):3363-3371.

Published online 9 Jun 2005

PMCID:PMC1148172.

© The Author 2005. Published by Oxford University Press. All rights reserved

() Detection of donor silent pilin copies using the SQ-PCR Av assay for strain FA1090 1-81-S2 grown for 22 h on solid media in the presence of IPTG. () Detection of donor silent pilin copies using the sequencing assay for strain FA1090 1-81-S2 grown for 22 h on solid media in the presence of IPTG. The parental copy sequence, , was retained in ∼97% of the templates in both assays, and the proportion of silent copy donors to the 3% of variant HV sequences is shown.