Analysis of genetic diversity between ivermectin responder phenotypes and drug-naïve (NTL) worms.

(A) Spearman rank correlation analysis of variant read frequencies from 248,102 SNPs. Values within each square represent the correlation coefficient for each pairwise analysis. (B) Degree of shared variation determined by pairwise comparisons of F_ST between treatment and response groups, summarised from 9,893 10-kb windows throughout the genome. F_ST distributions were compared statistically using a two-sample Kolmogorov-Smirnov [KS] test. (C) Variant read frequency spectrum from treatment and response subgroups for Cameroon and Ghana. The variant read frequency was calculated at each of the 248,102 SNP positions, from which the proportion of total variants in 0.05 frequency bins is presented. (D) Analysis of invariant loci per group as a proportion of the total number of variants observed, defined as variant read frequencies < 0.05 (blue) and > 0.95 (red).