Analysis of actin expression.
(A) HEK293T cells were mock-infected or infected with DENV 2 or DENV 4 and at 0, 20, 30 min and 1 hr p.i., cells were incubated with pan specific anti-dengue E protein antibody followed by an appropriate secondary antibody (green), phalloidin-TRITC (red) and DAPI (blue). Cells were observed under an Olympus Fluo View 1000 confocal microscope. Only the actin channel is shown. Full merged images with signal from all three channels are shown in Fig E in S1 File.