Analysis of DC surface markers and the cytokine secretion assay.

<p>(A) The immunophenotype of DCs pulsed with antigens. The mDC phenotypes were analyzed by flow cytometry using the indicated antibodies (black areas) and isotype controls (white areas). (B and C) IL-7 (B) and IL-12 (C) levels in DC culture supernatants, as determined by ELISA. The data are expressed as the mean±SD, and significant differences between the treated groups were detected using ANOVA followed by the Dunnett’s test. <sup>#</sup> <i>p</i><0.05 compared with the iDCs.</p>