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An Artificial Pathway for Isoprenoid Biosynthesis Decoupled from Native Hemiterpene Metabolism
journal contribution
posted on 2019-01-16, 04:29 authored by Sean Lund, Rachael Hall, Gavin J WilliamsIsoprenoids are constructed
in nature using hemiterpene building
blocks that are biosynthesized from lengthy enzymatic pathways with
little opportunity to deploy precursor-directed biosynthesis. Here,
an artificial alcohol-dependent hemiterpene biosynthetic pathway was
designed and coupled to several isoprenoid biosynthetic systems, affording
lycopene and a prenylated tryptophan in robust yields. This approach
affords a potential route to diverse non-natural hemiterpenes and
by extension isoprenoids modified with non-natural chemical functionality.
Accordingly, the prototype chemo-enzymatic pathway is a critical first
step toward the construction of engineered microbial strains for bioconversion
of simple scalable building blocks into complex isoprenoid scaffolds.
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non-natural chemical functionalityprenylated tryptophanArtificial Pathwayalcohol-dependent hemiterpene biosynthetic pathwayIsoprenoid Biosynthesis Decoupledprototype chemo-enzymatic pathwayisoprenoid scaffoldshemiterpene building blocksNative Hemiterpene Metabolism Isoprenoidsextension isoprenoidsnon-natural hemiterpenesscalable building blocksisoprenoid biosynthetic systemsprecursor-directed biosynthesis
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