Amino acids 81 and 82 of the mouse RNase L are also necessary for interaction with and inhibition by L*.
2018-04-13T17:45:48Z (GMT) by
<p>A. Segments of decreasing size were swapped between rat and mouse RNase L and the resulting chimeric RNase L were tested for inhibition by and interaction with L*<sub>DA</sub> (mouse virus) and L*<sub>RTV-1</sub> (rat virus)<sub>.</sub> Schematic rat/mouse chimeric RNase L are represented as in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1006989#ppat.1006989.g002" target="_blank">Fig 2</a>. The right columns indicate whether L*<sub>DA</sub> and L*<sub>RTV-1</sub> interact with and inhibit the chimeric enzymes. B-C. Immunoblots (B) show Flag and HA detection after immunoprecipitation of HA-L* (IP:HA) and in cell lysates (Input). Graphs (C) show the mean and SD of the amount of co-immunoprecipitated RNase L chimera relative to that of WT RNase L of the corresponding species (n = 3). *: p<0.05 in a two-way ANOVA followed by Dunnett’s test for multiple comparison. D. Analysis of RNase L-mediated RNA degradation in HeLa-M cells overexpressing indicated Flag-RNase L and L*<sub>DA</sub> or L*<sub>RTV-1</sub>. RNA samples were extracted 7 hours after polyI:C transfection. Arrowheads point to rRNA cleavage products. Reproducible results were obtained in 2 independent experiments.</p>